Description

Introduction

The term “chromatography” comes from the Greek terms “Chroma,” which means “colour,” and“graphien,” which means “to write.” Russian botanist M. S. Tswett invented the method at the beginning, in 1903. It is a method of analytical analysis Used to separate, identify and purify mixture’s constituents(Bachhav et al., 2023).It operates on the theory of differential interaction between Solutes in the stationary and mobile phases, which are two Separate phases. To address issues with analysis time and the Variety of substances that might be detected, many changes Were made to the chromatography procedures. Pumps were Used to practice applying pressure in order to shorten the run Duration. To improve detection, tools including spectroscopy and electrochemical techniques were included (Coskun, 2016).

The work of Archer John Porter Martin and Richard Laurence Millington Synge during the 1940s and 1950s, for Which they were awarded the 1952 Nobel Prize in Chemistry, greatly advanced the technology of chromatography. Their work promoted the quick development of Various chromatographic techniques, including paper Chromatography, gas chromatography and what would later Be known as high-performance liquid chromatography. They pioneered the fundamental concepts and procedures of partition chromatography. Since then, technological Development has accelerated. The various types of Chromatography mentioned below were developed as a result of researchers discovering that the fundamental ideas Behind Tsvet’s chromatography could be utilized in numerous Different ways. Chromatography’s technical performance is Always being improved, enabling the separation of molecules That are getting closer in similarity (Sowmya et al., 2022).